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flotation method for nematodes
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(PDF) Methods of preservation and flotation for the ...
The objective of this study was to evaluate preservation and flotation methods for the detection of nematode eggs and coccidian oocysts in faeces of the forest musk deer. The McMaster technique was...
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Efficiency of Extraction of Nematodes by Flotation-Sieving ...
extraction of nematodes from soil, compared to the sieving method of Cobb (9) or the Baermann funnel (9). The centrifugal- flotation method (4) reduced the time of operation required by other methods, but did not significantly affect the efficiency of extraction for most nematode species.
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PM 7/119 (1) Nematode extraction - 2013 - EPPO
2013-11-19 3.5 Centrifugal flotation. This method is used in other areas of biology and was adapted for the extraction of nematodes by Caveness Jensen . It is the only method that allows extraction of motile and immotile nematodes from soil.
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A COMPARISON OF METHODS FOR EXTRACTING
sieving and centrifugal flotation (SCF) is the most popular method and, presumably therefore, one of the more efficient methods used by contributors to this journal for extracting nematodes from soil. This technique was used on 36% of 186 occasions when a method was referred to for quantitative work
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Jenkins, W.R. (1964) A Rapid Centrifugal-Flotation ...
Jenkins, W.R. (1964) A Rapid Centrifugal-Flotation Technique for Separating Nematodes from Soil. Plant Disease Reporter, 48, 692. has been cited by the following article: TITLE: Influence of Tillage and Deep Rooted Cool Season Cover Crops on Soil Properties, Pests, and Yield Responses in Cotton
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(PDF) A COMPARISON OF METHODS FOR
The other methods tested were the Baermann tray, the two-flask sedi- mentation technique and direct centrifugal sugar flotation. Greater numbers of nematodes were extracted from chopped sugarcane...
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Nematode Extraction Techniques - Microbiology -
2021-1-6 Passive methods include filtration, or decanting and sieving, and flotation/centrifugation (Coleman et al., 1999) to remove the nematodes from the soil suspension. Elutriation methods can be employed for handling larger quantities of soil, usually greater than 500 g, or to recover large amounts and a greater diversity of nematodes.
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A rapid centrifugal-flotation technique for separating ...
, l litre water), thoroughly mixing sugar solution and sediment; the tubes are spun for 0.5 to 1 min. The supernatant containing the nematodes is poured on to a 325-mesh sieve. After rinsing the sugar away with water the nematodes are ready for use.
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A method for the quantitative extraction of
In this study of the optimum conditions for the extraction of all stages of Meloidogyne spp. from hard and soft root tissues, experiments showed that time of maceration and of centrifugal flotation are of great importance. Best results were obtained when prepared roots were macerated in a Waring blendor at the lowest speed for 15 seconds for the extraction of swollen stages and 120 seconds for...
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Extraction of Nematodes by Decanting and Sieving
2020-1-1 Procedure. 1. Review potential extraction errors. 2. Mix soil sample and pass through coarsesieve to remove rocks, roots, etc. 3. Take a 250 cc subsample of soil: packlightly into beaker for uniformity. 4. Place soil in one of the 2 liter pitchers;half fill with water.
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Efficiency of Extraction of Nematodes by Flotation-Sieving ...
two flotation solutions on the same day using the standard procedure. The soil samples chosen contained a very wide range of both numbers and kinds of nematodes. A smaller number of soil samples was used in comparisons with the elutriation method. Soil samples of 450 cc were used for elutriation.
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A COMPARISON OF METHODS FOR EXTRACTING
flotation method the most efficient for clay soils. The other methods tested were the Baermann tray, the two-flask sedi- mentation technique and direct centrifugal sugar flotation. Greater numbers of nematodes were extracted from chopped sugarcane roots incubated in a polythene bag in hydrogen peroxide solution than when incubated in water. ...
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Double layers centrifugal-flotation method for the ...
DOI: 10.1303/JJAEZ.14.108 Corpus ID: 88193439. Double layers centrifugal-flotation method for the separation of nematodes @article{Takagi1970DoubleLC, title={Double layers centrifugal-flotation method for the separation of nematodes}, author={K. Takagi}, journal={Japanese Journal of Applied Entomology and Zoology}, year={1970}, volume={14}, pages={108-110} }
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Jenkins, W.R. (1964) A Rapid Centrifugal-Flotation ...
Jenkins, W.R. (1964) A Rapid Centrifugal-Flotation Technique for Separating Nematodes from Soil. Plant Disease Reporter, 48, 692. has been cited by the following article: TITLE: Influence of Tillage and Deep Rooted Cool Season Cover Crops on Soil Properties, Pests, and Yield Responses in Cotton
Read More
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EVALUATION OF METHODS FOR EXTRACTION OF
2019-3-1 and sieving, the Whitehead direct method (Whitehead tray), and centrifugal-flotation with silica (Ludox LS), sucrose and MgSO4, respectively. The most efficient methods for non-parasitic nematodes were centrifugal-flotation with silica, the Seinhorst elutriator and the Whitehead tray.
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Isolation of C. elegans and related nematodes
2013-6-28 4. Centrifugal flotation Terrestrial nematodes have a specific gravity of 1.08 and can be recovered by flotation on a solution with a specific gravity of 1.15 (Hooper, 1986). This method is best used with soil samples, whereas decaying leaves or compost material yield too much detritus. Several solutions can be used:
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Nematode Extraction Techniques - Microbiology -
2021-1-6 Nematodes may be extracted by a variety of techniques, either active or passive in nature. The principal advantage of the oldest, active method, namely the Baermann funnel method, is that it is simple, requiring no sophisticated equipment or electricity. It is
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Methods for extracting plant pathogenic nematodes from ...
2015-9-1 A new method for treating nematode infested Brachiaria seeds is being devel-oped by Incotec Sweden AB, Uppsala. To be able to assure that the seeds are free from living nematodes after treatment, a method for control of the treated seeds is needed. This method needs to be able to separate living nematodes from dead
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Evaluation and application of a molecular method to
2011-7-1 1. Introduction. Strongylid nematodes of ruminants represent a major financial burden on the livestock industry worldwide. These parasites are responsible for substantial economic losses due to the diseases that they cause and the costs associated with their treatment and control (e.g., Perry and Randolph, 1999, Sackett et al., 2006).Most commonly, small ruminants, such as sheep, are affected ...
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Nemaplex
2021-4-13 A Virtual Encyclopedia on Soil and Plant Nematodes . Enter Nemaplex. Note: If the main dropdown menu is not working with Chrome: Use Microsoft Edge . N E M A P L E X. is developed and maintained by: Howard Ferris. Department of Entomology and Nematology
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Efficiency of Extraction of Nematodes by Flotation-Sieving ...
two flotation solutions on the same day using the standard procedure. The soil samples chosen contained a very wide range of both numbers and kinds of nematodes. A smaller number of soil samples was used in comparisons with the elutriation method. Soil samples of 450 cc were used for elutriation.
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An improved method for the extraction of nematodes
The Baermann funnel method could only recover live nematodes; however, it takes longer. Sucrose solutions as density-gradient media for centrifugal flotation technique have proven to be valuable tools to isolate nematodes quickly, making the centrifugal flotation method one of the most preffered methods for the extraction of nematodes.
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Double layers centrifugal-flotation method for the ...
DOI: 10.1303/JJAEZ.14.108 Corpus ID: 88193439. Double layers centrifugal-flotation method for the separation of nematodes @article{Takagi1970DoubleLC, title={Double layers centrifugal-flotation method for the separation of nematodes}, author={K. Takagi}, journal={Japanese Journal of Applied Entomology and Zoology}, year={1970}, volume={14}, pages={108-110} }
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EVALUATION OF METHODS FOR EXTRACTION OF
2019-3-1 and sieving, the Whitehead direct method (Whitehead tray), and centrifugal-flotation with silica (Ludox LS), sucrose and MgSO4, respectively. The most efficient methods for non-parasitic nematodes were centrifugal-flotation with silica, the Seinhorst elutriator and the Whitehead tray.
Read More
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Nematodes - Van Gundy - 1983 - Agronomy
Sugar‐flotation method is a rapid, simple processing technique used in many laboratories to recover all nematodes, dead or alive, in soil samples. Since most soil nematodes are transparent and lesser than 2 mm long, counting is usually carried out at x 20 to 100 magnification on a
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CC/NUMBER 41 OCTOBER 13, 1980 This Week's Citation
2004-4-22 A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Dis. Rep. 48:692, 1964. [Dept. Entomology and Economic Zoology, Rutgers University, New Brunswick, NJ] This paper presented a simple, rapid, and inexpensive method for the extrac-tion of nematodes from soil. It used readily available equipment and was
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NEW TECHNIQUES FOR COUNTING AND ISOLATING
In the identification of the nematodes mounted in lactophenol the phase contrast microscope is a valuable help. b. The centrifugal flotation method The staining technique is very valuable as a method for checking the relability of other techniques but it takes to much time to be used
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Nematode Extraction Techniques - Microbiology -
2021-1-6 Nematodes may be extracted by a variety of techniques, either active or passive in nature. The principal advantage of the oldest, active method, namely the Baermann funnel method, is that it is simple, requiring no sophisticated equipment or electricity. It is
Read More
-
Methods for extracting plant pathogenic nematodes from ...
2015-9-1 A new method for treating nematode infested Brachiaria seeds is being devel-oped by Incotec Sweden AB, Uppsala. To be able to assure that the seeds are free from living nematodes after treatment, a method for control of the treated seeds is needed. This method needs to be able to separate living nematodes from dead
Read More
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